Inverter

Part:BBa_K3252043:Design

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


dCas9-GFPmut3b Inverter Module


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1447
    Illegal NheI site found at 1206
    Illegal NheI site found at 4373
    Illegal NheI site found at 4396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1447
    Illegal BamHI site found at 3485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5071


Design Notes

This part was assembled with BioBricks assembly method using XbaI restriction enzyme.


Source

This part sequence was adapted from this reference.

Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283

References